A metal and enamel device 1 1/4 inches (3.18 cm) in height overall consisting of a gold colored textured Norman shield displaying a diagonally placed buff strip from upper left to lower right between two narrow diagonally placed blue strips, one on each side, all between in the upper right section a pack mule and in lower left a wheel, all black, all within a narrow gold colored textured border, bearing fourteen gold disks.
The insignia is the shield of the First Cavalry Division Train. Buff is the color traditionally associated with the Quartermaster Corps, while yellow and blue refer to the affiliation with the 1st Cavalry Division. The pack mule and wagon wheel represent methods of past and present transport utilized by the organization.
The distinctive unit insignia was originally approved for the First Cavalry Division Train, Quartermaster Corps on 14 February 1925. It was redesignated for the 16th Quartermaster Squadron effective 1 April 1936. The insignia was rescinded on 1 April 1958.